C-myc epitope tag antibody
WebBy Stewart Newlove, PhD. Epitope tags are short peptide sequences, generally composed of 10-15 amino acids, designed to create a molecular handle for a protein. An epitope tag can be placed anywhere within a protein but typically are placed on either the C-terminus or N-terminus in order to minimise tertiary structure disruptions that may alter ... WebAnti-Myc has utility to detect the fusion protein of the Myc epitope cloned along with the target gene. As such, anti-Myc/Myc can be used to identify fusion proteins containing the …
C-myc epitope tag antibody
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WebImmunogen: This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide, E-Q-K-L-I-S-E-E-D-L, conjugated to KLH using maleimide. The sequence corresponds to amino acids 410-419 of human c-Myc. WebStudy c-Myc binding partners and target genes with our highly validated antibodies and high purity inhibitors. MAX antibodies and inhibitors: Mouse monoclonal [73C5a] to MAX - ChIP Grade ab53570. Rabbit polyclonal to Max (phospho S11) ab191493. Cell permeable c-Myc-Max dimerization inhibitor (10058-F4) ab145065.
WebAn epitope located within amino acids 410-419, containing the sequence EQKLISEEDL of human c-Myc has been widely used as a tag in many expression vectors, enabling the expression of proteins as c-Myc tag fusion proteins. Epitope tags provide a method to localize gene products in a variety of cell types, to study the topology of proteins and ... WebWhat are epitope tags? Antibodies are an essential tool to determine the localization and expression of target proteins, and for purification in the presence of numerous other proteins in living organisms. ... c-Myc protein is a transcription factor involved in the cell cycle and apoptosis. Abnormalities in c-Myc, such as mutations and ...
WebMonoclonal antibodies are produced by injecting an antigen into a host animal, in order to initiate a humoral immune response, and by subsequently extracting the antibody-producin WebMyc Tag Monoclonal Antibody (Myc.A7), DyLight™ 488, Invitrogen™-Mouse Monoclonal Antibody Achetez Myc Tag Monoclonal Antibody (Myc.A7), DyLight™ 488, Invitrogen™
WebMyc Tag Antibodies. The Myc tag, derived from the c-Myc protein, is a popular epitope tag for detecting the expression of recombinant proteins in yeast, bacteria, insect, and mammalian cell systems. The Myc tag may be fused to either the N-terminus or C … Find the Myc Tag antibody that fits your needs. Choose from 1 of 77 Myc Tag …
WebSep 27, 2024 · Epitope tags are widely used in various applications, but often lack versatility. ... Ramsay, G. & Bishop, J. M. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product ... how to install startpage on microsoft edgeWebMar 22, 2024 · One of the most widely used epitope tags is the myc-tag, recognized by the anti-c-Myc hybridoma antibody Myc1-9E10. Combining error-prone PCR, DNA … how to install starter shims on chevy trucksWebThe 9E10 antibody epitope (EQKLISEEDL) derives from a protein sequence in the human proto-oncogen p62(c-myc) and is widely used as a protein fusion tag. This myc-tag is a powerful tool in protein localization, immunochemistry, ELISA or protein purification. Here, we characterize the myc-tag epitope by substitutional analysis and length ... how to install starter on 350 chevyWebThe mouse Anti-c-MYC monoclonal antibody recognizes an epitope located within a sequence (EQKLISEEDL) of the product of the human oncogene c-myc. c-Myc (v-myc avian myelocytomatosis viral oncogene homolog) is a transcription factor, which is coded by MYC gene. It was first described as a homolog of an avian retroviral oncogene. how to install start button windows 10WebEpitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag ... how to install startup show app on firestickWebWestern blot. Left Lane -- Cultured normal mouse embryo fibroblasts were transfected with a plasmid containing the SAP1a cDNA fused with a c-myc epitope tag at its C-terminus. Lane 1, 30 µg protein; lane 2, 20 µg protein; lane 3, 10 µg protein; lane 4, 5 µg protein. how to install starter shingles roofWebWhether you’re studying endogenous c-Myc or Myc-tagged proteins, aim for perfection with our highly validated Myc antibodies. Mouse monoclonal antibody (9E10 clone) to the Myc tag to study Myc-tagged proteins. Rabbit monoclonal antibody (Y69) to c-Myc to specifically detect endogenous c-Myc. Both antibodies are: Rated as excellent by users ... joosenhouse led vanity light