WebThe primary data for published Broad Institute ChIP-Seq experiments have been deposited to the NCBI GEO database under the following accessions: Mikkelsen et al. (2007): … WebRabbit Polyclonal SMYD3 antibody. Validated in WB, ICC/IF, IP, ChIP assay. Tested in Human, Mouse. Cited in 5 reference(s). Independently reviewed in 3 review(s). ... Cross-linked ChIP was performed with HepG2 chromatin extract and 5 μg of either normal rabbit IgG or anti-SMYD3 antibody. The precipitated DNA was detected by PCR with primer …
Anti-SMYD3 ABE2870
WebHistone methylation marks in the genome can be mapped by ChIP-seq. Different types of regulatory DNA sequences (e.g., promoter and enhancer) are often marked by specific histone methylation states or a combination of histone modifications. ... In this study, they observed that a SNP in the regulatory region of SMYD3 creates a third E2F binding ... WebUsing chromatin immunoprecipitation coupled with deep sequencing (ChIP-seq) analysis, PARP16 was identified as a novel target gene for histone H3 lysine 4 (H3K4) methyltransferase SMYD3, and SMYD3 ... download ams
H3K4 Methyltransferase Smyd3 Mediates Vascular …
WebMar 21, 2024 · SMYD3 (SET And MYND Domain Containing 3) is a Protein Coding gene. ... Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for … WebJul 8, 2024 · And, PCR and Western blot were used to verify their expression. The binding of SMYD3 on the promoter of target genes were tested by ChIP assays. We also use nude mice subcutaneous tumor model and patient-derived xenograft (PDX) model to investigate the tumor promotive function of SMYD3 in vivo. WebMechanistically, Smyd3 directly bound to the promoter region of Cdkn1a (coding for p21), then caused its increased H3K4me3 level and elevated gene expression, and ultimately gave rise to senescence-associated phenotypes. Intriguingly, Smyd3-mediated p21 upregulated expression also exists in human tissues of vascular disease, indicating it is ... download am smartmeet