WebOur dual fluorescent dextran derivatives are produced by conjugating two fluorophores, namely FITC and TRITC as well as FITC and AR onto a dextran molecule through thiourethane linkages. The degree of substitution (DS) lies in the range of 0.001 to 0.020 mol of dye per mol of glucose units for each of the fluorophores. Read more WebRhodamines are xanthene derivatives structurally related to fluorescein, but with additional chemical substitutions that shift their excitation and emission spectra to longer wavelengths.The most widely used rhodamines include rhodamine 110, the parent rhodamine, tetramethylrhodamine (such as TAMRA or TRITC) and X-rhodamines (such …

Triple Band Excitation: DAPI-FITC-TRITC Nikon’s MicroscopyU

WebFigure 1 below illustrates the excitation and emission spectra of FITC under conditions of 488 nm excitation. FITC’s emission maximum is around 530 nm. This means that if we excite this molecule, there is a very high … http://docs.abcam.com/pdf/immunology/fluorochrome_guide.pdf sql date format yyyy-mm-dd hh:mm

FITC-labelled polysaccharides - Sigma-Aldrich

WebAug 22, 2024 · The excitation wavelengths were λ = 561 nm for TRITC and λ = 491 nm for GFP and fluorescein. The excitation objective was a 10×, 0.3 NA Plan Fluor from Nikon, while the detection objective was ... WebThe most common members of this group have been the tetramethylrhodamines—including the reactive isothiocyanate (TRITC) and carboxylic acid (TAMRA) derivatives—as well as the X-rhodamines. The X prefix of the X-rhodamines, ... fall between those of fluorescein and tetramethylrhodamine (Figure 1.6.3). With a peak absorption at ~520 nm, ... WebIn fact, the fluorescein and rhodamine probes have been so widely used that most microscope and aftermarket filter manufacturers have specialized filter sets named after their reactive isothiocyanate intermediates (FITC … sql date format without 0