Guava nexin assay
WebOct 15, 2008 · Cell proliferation assay. The in vitro cytotoxicity was determined using 3- (4,5-dimethylthiazol-2-yl)-5- (3-carboxymethoxyphenyl)-2- (4-sulfophenyl)-2H-tetrazolium (MTS), which is chemically reduced by metabolically active cells into formazan, and the intensity of the resulting color is proportional to the number of living cells. CDC. WebJun 20, 2008 · The new mix-and-read Guava Nexin assay relies on a two-dye strategy. The assay uses Annexin V-PE to detect the translocation of phosphatidylserine to the …
Guava nexin assay
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WebNov 27, 2024 · 632844 The Guava Nexin Reagent is a mix-and-read assay for monitoring externalization of phosphatidylserine (PS) through the binding of Annexin V to the exposed PS. I have used this reagent for measuring levels of Annexin V in our control and test samples. Our results was good and showed difference between the group. WebCell viability, migration, invasion and apoptosis were measured using CCK-8 assay, two-chamber migration (invasion) assay and Guava Nexin assay, respectively. The …
WebMethods: Cell transfection was conducted to change the expression of THRIL and microRNA-125b (miR-125b) in ATDC5 cells. qRT-PCR was performed to detect the expression of THRIL, miR-125b and pro-inflammatory cytokines IL-6, TNF-α and monocyte chemotactic protein 1 (MCP-1) in ATDC5 cells. WebThe GuavaEasyCyte 5 flow cytometer is a simple instrument used for fluorescent cell and particle counting. This is a micro capillary based system that is easy to use. The micro …
WebNov 1, 2013 · The impact of geranylgeraniol and lovastatin (Lova) on the initiation of apoptosis in human DU145 prostate carcinoma cells. (a) DU145 cells were incubated with geranylgeraniol (0, 50, and 100 μmol/L) and lovastatin (10 μmol/L) for 24 h and then analyzed by flow cytometry using the Guava Nexin ™ Assay. Cells in the lower left … WebFeb 1, 2008 · The Guava Nexin assay (Part Number 4500-0161) was performed following the manufacturer's instructions. Briefly, 3.0 × 10 4 cells (50 μL) were added to a 150 μL staining solution containing 135 μL 1× apoptosis buffer, 10 μL Annexin V …
WebDec 1, 2024 · Guava Nexin assay (4500-0450, Guava Technologies, CA, USA) was utilized for measuring HaCaT cell apoptosis as earlier described [22]. Briefly, cells were …
WebDec 1, 2024 · Guava Nexin assay (4500-0450, Guava Technologies, CA, USA) was utilized for measuring HaCaT cell apoptosis as earlier described [22]. Briefly, cells were cultivated into 6-well plate and subjected to relevant treatment. guiding beacon pathfinderWebJun 23, 2024 · MTT assay showed that ABT199 + AZD5 displayed a similar synergistic effect on H1975AR compared with ABT263 + AZD5. Flow cytometry analyses showed that ABT263 + AZD5 had higher cell populations of early apoptotic and apoptotic in Nexin assay as well as higher cell population arrested at pre-G1 phase representing apoptotic cells … guiding beacon pathfinder kingmakerWebNov 27, 2024 · 632844 The Guava Nexin Reagent is a mix-and-read assay for monitoring externalization of phosphatidylserine (PS) through the binding of Annexin V to the … bourbon pronounceWebJun 11, 2009 · Complement-dependent cytotoxicity (CDC) and antibody-dependent cellular cytotoxicity (ADCC) assays were performed using Daudi cells as described previously. 9 Blood specimens were collected under a protocol approved by the New England Institutional Review Board (Wellesley, MA), and informed consent was obtained in accordance with … bourbon pronunciationWebDescription Guava Nexin Reagent for Flow Cytometry - 100 tests Overview The Guava Nexin® Annexin V Assay offers a simple, reproducible, and reliable assessment of early … bourbon pound cake southern livingWebDec 17, 2024 · The results obtained with the Guava Nexin assay showed similar behavior for all the substances analyzed except for 2C-B. In fact, 2C-B stimulated the apoptosis of TK6 cells only at the highest concentrations tested (25 and 35 µM). This fact is of crucial toxicological importance and has great repercussions in terms of genotoxicity, because it ... bourbon profilesWebFewer than 2 x 104 cells in 100 µL original volume are required for the mix-and-read Guava Nexin Assay. The cells are stained directly in the 96-well microplate or tube with Guava … bourbon propane