NettetHoechst 33342 is a well-characterized blue-emitting fluorescent compound widely utilized for nuclear staining. Hoechst 33342 permeates cell membranes and binds to the minor … NettetHoechst 33342 is a well-characterized blue-emitting fluorescent compound widely utilized for nuclear staining. Hoechst 33342 permeates cell membranes and binds to the minor groove of A/T-rich dsDNA sequences, thus preferentially staining nuclei (Figure 1). Fig.1. Hoechst 33342 (in blue) binding dsDNA. Hoechst 33342 Excitation / Emission Spectra

Hoechst 33258 Staining Dye Solution (ab228550) Abcam

NettetHoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed pycnotic nuclei in apoptotic cells and for cell cycle studies in combination with BrdU. It is also available in solution (H-3570). NettetPureBlu Hoechst 33342 Nuclear Staining Dye is a highly pure formulation of Hoechst 33342 fluorescent dye ... PureBlu™ Hoechst 33342 Nuclear Staining Dye Bio-Rad Laboratories, Inc. 2000 Alfred Nobel Drive, Hercules, CA 94547 510-741-1000 10043283 Rev A 14-1036 0614 final stage of clotting process

The Use of Hoechst Dyes for DNA Staining and Beyond - MDPI

NettetHoechst 33342 is a cell-permeable fluorescent compound that is able to stain the DNA of eukaryotic and prokaryotic cells by binding with high affinity to the minor groove of AT … Nettet1. okt. 2015 · Figure 2: Live-cell imaging with SiR–Hoechst. ( a) Long-term live-cell microscopy with SiR–Hoechst and other far-red DNA dyes. HeLa cells were imaged for 24 h on a wide-field epifluorescence ... Nettet5. Incubate the blood and Hoechst stain for 5–10 min at room temperature to allow for fluorescent staining of cell nuclei. 6. Extremely gently, pipette 1.5 μL of stained blood into the loading chamber using a gel-loading tip (Eppendorf), taking care to draw the tip out of the device while dispensing. gshock london