Mouse brain paraffin embedding protocol
NettetEmbedding is important in preserving tissue morphology and giving the tissue support during sectioning. Some epitopes may not survive harsh fixation or embedding. The tissue is typically cut into thin sections (5-10 µm) or smaller pieces (for whole mount studies) to facilitate further study. Paraffin-embedded tissue. Nettet1. des. 2024 · Between each step of the standard paraffin embedding protocol, the mouse brain was placed in an Eppendorf tube filled with the embedding media and …
Mouse brain paraffin embedding protocol
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Nettet23. jul. 2024 · TBA-Dehydrating Paraffin Embedding. For mouse brains, the brain was first removed from the skull and transferred to neutral buffered PFA (4% w/v in 10 mM … NettetAfter perfusion fixation and paraffin embedding, 3-μm thick sections were cut and stained with Nissl or Haematoxylin & Eosin (H&E) staining using standard histological protocol as described earlier (Sharma et al., 1990b, 1993; Zimmer et al., 1991).
Nettet9. nov. 2024 · Affiliations. 1 Basic Medical Science Department, College of Medicine, QU Health, Qatar University, Doha, Qatar. 2 Charité - Universitätsmedizin Berlin, Institute of Cell and Neurobiology, Berlin, Germany. 3 Charité - Universitätsmedizin Berlin, Center for Chronically Sick Children (Sozialpädiatrisches Zentrum, SPZ), Berlin, Germany. NettetConfocal immunofluorescent analysis of fixed frozen wild-type mouse brain using Cathepsin D (E7Z4L) XP ® Rabbit mAb (green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, the tissue was then labeled using Iba1/AIF-1 (E4O4W) XP ® Rabbit mAb (Alexa Fluor ® 555 …
Nettet1. jan. 2013 · In current neuroscience studies, paraffin embedding and freezing formaldehyde-fixed tissue are the most widely used preparations for LM studies. Gelatin or agarose embedding of Golgi’s stained tissue, embryos, and newborn tissues for vibratome cutting are still widely in use today. NettetAmong the five somatostatin receptors (SST1–SST5), SST4 is the least characterized, which is in part due to the lack of specific monoclonal antibodies. We generated a knockin mouse model that expresses a carboxyl-terminal SST4-eGFP fusion protein. In addition, we extensively characterized the novel rabbit monoclonal anti …
Nettet10. okt. 2024 · Paraffin-embedded section; Heparan sulfate 3-O-sulfotransferase; ... In this chapter, we present a detailed protocol for in situ hybridization analysis of the expression of HS 3-OST in mouse brain (Fig. 1). ... Mouse brain cDNA library (Takara Bio Inc., Otsu, Japan). 2.
Nettet1. jan. 2013 · Cool the paraffin-embedded tissue by attaching coolant against the surface of the block; this is a key step to cutting a good section. Either applying ice wrapped in … cisco catalyst 2960s 24td lNettetIn pathology protocols, a tissue block, such as one containing a mouse brain or a biopsy sample from a patient, can produce several hundred thin sections. cisco catalyst 2960 sfpNettetIncubation time. 10% Sucrose. 15 min or until sample drops to the bottom of the vial. 30% Sucrose. 15 min or until sample drops to the bottom of the vial. Partially fill dry ice … cisco catalyst 2960x datasheetNettet28. jul. 2024 · The entire protocol took about 2-30 days to complete for a large sample, including fixation, dehydration, clearing, wax immersion and embedding. In addition, it could be applied to other species and organs, while the embedding processes depended on the size and the type of organs. cisco catalyst 2960 manualNettetThis is dependent on the details of your experiment. Formalin-fixed paraffin-embedded tissue can be stored long term at room temperature whereas frozen tissue can only be … cisco catalyst 2960x 48lps l specsNettetI am a research scientist passionate about finding and promoting innovative solutions for human health problems. I have 15+ years of … cisco catalyst 2960 ws c2960 24tc lNettet14. mar. 2024 · Embedding and Chemical Reactivation of Green Fluorescent Protein in the Whole Mouse Brain for Optical Micro-Imaging Embedding and Chemical Reactivation of Green Fluorescent Protein in the Whole Mouse Brain for Optical Micro-Imaging Front Neurosci. 2024 Mar 14;11:121. doi: 10.3389/fnins.2024.00121. eCollection 2024. Authors cisco catalyst 2960-xr