WebSimilarly, age and gender-matched Myh6- MerCreMer+; Tead1W/Wmice were used as an additional control.All 3 groupsofmicewere receivedTAM injection in the same way as for igKO mice except with lower... Web27 nov. 2024 · In this system, Myh6-MerCreMer:R26R-Confetti-labelled cells and their daughter cells could express one of four different fluorescent reporters: nGFP, YFP, RFP or mCFP. Fig. 4 Gel–miR-302-induced...
RNA-binding protein QKI governs a muscle-specific alternative …
WebMice bearing either floxed Ulk1 or floxed Ulk2 alleles were crossed with transgenic mice expressing a tamoxifen-inducible Cre recombinase (Myh6-MerCreMer). Gene … WebExpression of Myh6 was unaffected by miR302-367 overexpression ( Fig. 3E ). Overexpression of miR302-367 in developing cardiomyocytes therefore led to a highly proliferative, immature dedifferentiated phenotype in cardiomyocytes. In contrast, miR302-367 overexpression resulted in down-regulation of programmed cell death ( Fig. 3D ). creed bike lights
Generation of Myh6MerCreMer/+ knock-in mouse. (A
Webreferred to as MerCreMer (MCM), is expressed[4,5]. Administration of tamoxifen (TAM) or 4-hydroxytamoxifen activates the MCM protein, leading to its nuclear translocation and … Web17 mrt. 2024 · In fact, Zhang et al. exploited this aspect of cardiomyocyte response to injury in their experimental strategy to identify “dedifferentiating” cardiomyocytes, noting that … Web1 jul. 2011 · To circumvent this limitation, a widely used inducible cardiac-specific model, Myh6 -MerCreMer (Cre), using tamoxifen (TAM) to activate Cre has been developed. The current study examined cardiac function in Cre-positive C57B/J6 mice exposed to one, three, or five daily doses of a 40 mg/kg TAM to induce Cre activity specifically in the heart. buckner\u0027s bourbon