Pinene synthase
WebS-limonene synthase is a model monoterpene synthase that cyclizes geranyl pyrophosphate (GPP) to form S-limonene. It is a relatively specific enzyme as the majority of its products are composed of limonene. In this study, we converted it to pinene or phellandrene synthases after introducing N345A/L4 … WebMar 18, 2024 · Pinene (C10H16) is a monoterpenoid compound with a molecular weight of 136.23 Da. According to its features of noncytotoxicity and tastelessness, pinene has been increasingly used in modern industries, such as rubber, coatings, printing, food packaging, and hygiene [ 1, 2 ].
Pinene synthase
Did you know?
WebDec 17, 2024 · The successful pinene production of transgenic Synechococcus was achieved at the highest rate of 1.525 ± 0.l45 mg L −1 under the condition of CO 2 aeration and continuous light. Furthermore, the ratio of two pinene isomers, α - and β -pinene, was also determined in the final product. Materials and Methods Strains and Construction of … WebAug 15, 2024 · We used the pipeline to screen pinene synthase variant libraries, with mutations in three areas of plasticity, capable of producing multiple monoterpene products.
WebMay 1, 2004 · cDNA isolation, functional expression, and characterization of (+)-α-pinene synthase and (−)-α-pinene synthase from loblolly pine Pinus taeda: stereocontrol in pinene biosynthesis. Archives of Biochemistry and Biophysics, 411 (2003), pp. 267-276. View PDF View article View in Scopus Google Scholar. WebCrystal structural data for (4S)-limonene synthase [(4S)-LS] of spearmint (Mentha spicata L.) were used to infer which amino acid residues are in close proximity to the substrate and carbocation intermediates of the enzymatic reaction. Alanine-scanning mutagenesis of 48 amino acids combined with enzyme fidelity analysis [percentage of (−)-limonene …
WebSep 12, 2024 · The C. sativa β-pinene synthase is as yet unidentified, but it is common for α-and β-pinene synthases in other species to produce some amount of β-myrcene as a by-product [ 20 ]. Cis-ocimene is produced as a single product by TPS13 [ 7 ], and shows no correlation to other terpenes ( Fig 3 ). WebHere, we provide an overview on the synthesis and metabolic pathways, regulatory pathways, and key enzymes in the biosynthetic pathways of terpenoids. This paper will …
WebApr 14, 1998 · These transformations catalyzed by the terpenoid synthases (cyclases) may be followed by a variety of redox modifications of the parent skeletal types to produce the …
WebSep 26, 2024 · The enzyme pinene synthase catalyzes the formation of either α- and/or β-pinene by cyclization of GPP. Pinene synthase is mostly found in plants, notably pine trees such as A. grandis, Pinus taeda, and Picea abies. Several pinene synthases have been characterized, with some producing both isomers of pinene (α and β), and others only … california small engine ban chainsawWebAug 29, 1997 · Pinene synthase has previously received considerable attention as a major defense-related monoterpene synthase in conifers (18, 19). In the grand fir cDNA library, which was synthesized from mRNA obtained from wound-induced sapling stems, clones corresponding to pinene synthase are at least 10 times more abundant than clones for … coast capital savings federalWebAug 24, 2024 · Here, we characterized two terpene synthase genes, α-pinene synthase ( PmTPS4) and longifolene synthase ( PmTPS21 ), identified in resistant P. massoniana … coast capital savings help headquartersWebDec 1, 2024 · IPP and DMAPP are synthesized via either the 1-deoxy- d -xylulose-5-phosphate (DOXP) pathway or the mevalonate (MVA) pathway and condensed to form the intermediates geranyl diphosphate (GPP), farnesyl diphosphate (FPP) and geranylgeranyl diphosphate (GGPP) ( Zebec et al., 2016 ). california small claims statutesWebMay 17, 2016 · In this context, the objectives of present study were to identify, isolate, and functionally characterize α-pinene synthase genes from P. pinaster and P. pinea using … california small engine ban generatorWebMay 31, 2024 · The fluidities and permeabilities of cell membranes of Candida glycerinogenes decrease as pinene levels increase resulting in adverse effects on cell growth, and the biomass of yeast cells cultivated in shake flask systems are improved by exogenous addition of trehalose and ergosterol. Pinene is a commercially important … california small claims timelineWebAug 30, 2024 · Pinene is an active natural monoterpene from plants and has important applications in flavorings, fragrances,and pesticides. Especially, pinene dimers are regarded as renewable fuels with high density. However, the microbial pinene production was limited by the low activity pinene synthase. In this study, the pinene synthase activity was … california small court claims