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WebAvoid direct repeats in the primers to prevent misalignment in binding to the target. Consider longer primers to enhance specificity. Consider nested PCR to improve specificity. High quantity: Optimize primer concentrations (usually in the range of 0.1–1 μM). High primer concentrations promote primer-dimer formation. Other reaction components WebApr 10, 2024 · The perinucleolar compartment (PNC) is a small nuclear body that plays important role in tumorigenesis. PNC prevalence correlates with poor prognosis and cancer metastasis. Its expression in pediatric Ewing sarcoma (EWS) has not previously been documented. In this study, we analyzed 40 EWS tumor cases from Caucasian and … WebWhy? b) which test would likely benefit most from a longer incubation time? Why?, Ex. 5-4 (Methyl Red/VP) Would a false negative result for the VP test more likely be attributable to … sunshine hills elementary school