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RecA and RecB: probing complexes of DNA repair proteins with
WebbThe RecBC complex requires the RecD subunit for nuclease activity, but can translocate along ssDNA in both directions. Probably interacts with a component of retron Ec48 … Webb17 feb. 2006 · Because RecB nuc is a stable structural domain with a defined nucleolytic function, it was surprising that this domain was also implicated, by deletion analysis, in RecA loading. To determine whether this region is indeed involved in RecBCD-facilitated loading of RecA, we purified 6×His-tagged RecB nuc and investigated its ability to … ti-84 graphing calculator download
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Webb4 okt. 2024 · The RecA protein and RecBCD complex are key bacterial components for the maintenance and repair of DNA, RecBCD a helicase-nuclease that uses homologous … WebbExonuclease V, a RecBCD complex from E. coli has several different enzyme activities, including an ATP-dependent single-stranded DNA endonuclease activity, ss- and ds- DNA exonuclease activity. The hydrolysis in each case is bi-directional (from both the 3´ and 5´ ends) and processive, producing oligonucleotides (1,2,3). Webb6 juli 2024 · IECRE, the IEC System for Certification to Standards Relating to Equipment for Use in Renewable Energy Applications, has approved Lloyds Register (LR) as a … ti 84 graphing calculator ce