WebMay 18, 2012 · STAT1 Y701F and S727A mutants could be ubiquitinated and degraded by Smurf1 with the same efficiency as the WT STAT1. Thus, our findings suggest that Smurf1 is a cellular ubiquitin ligase with specificity toward unphosphorylated STAT1 proteins. Smurf1 plays essential roles in development . The possible role of the Smurf1 protein in … WebJul 1, 2008 · Analysis of Stat1(−/−) cells stably transfected with the GFP-NLS-S1 or GFP-NLS-S1-Y701F constructs revealed that GFP-NLS-S1 became S727-phosphorylated in response …
Kinetics of regulated protein–protein interactions revealed with ...
WebSTAT1 dimers, also called gamma interferon-activated factor (GAF), translocate to the nucleus and promote gene expression by binding to gamma interferon-activated site (GAS) of interferon-stimulated genes (ISG). On the other hand, stimulation with IFN-I or IFN-III leads to TYK2- and JAK1-mediated phosphorylation of STAT1 and STAT2. WebY701F mice To investigate STAT1 activity in the absence of its tyrosine phospho- rylation, we generated Stat1 Y701F knock-in mice by targeting the gene with a construct encoding phenylalanine in position 701 (see Materials and Methods). We first examined heterozygous Stat1 Y701F/+ mice. how to keep your skin young and healthy
An inhibitory effect on the nuclear accumulation of …
WebOct 15, 2000 · Stable transfectants constitutively expressing Stat1HA Y701F or Stat1HA were made by electroporation of U-937 cells (250V/960 μF). Selection with 1000 μg/mL of G418 (Life Technologies, Paisley, United Kingdom) started 48 hours after electroporation, and clones were collected about 3 weeks later. WebFeb 2, 2024 · STAT1 F77A/F77A mice exhibit a pronounced immune cell infiltration in the ischaemic heart. After the assessment of STAT1 and STAT3 levels and tyrosine … WebApr 1, 2024 · Download Citation On Apr 1, 2024, Andreas Begitt and others published A family-wide assessment of latent STAT transcription factor interactions reveals divergent dimer repertoires Find, read ... josephine\u0027s kitchen south padre island