Webb29 mars 2024 · We first compared the fluorescence intensity of Thy1-YFP and CRH-cre;Ai32 mice. At the same excitation intensity, the fluorescence signal of Thy1-YFP brain slices was very strong and clearly observed, in contrast to the ChR2-EYFP-labelled brain slices (Fig. 3A), indicating Webb20 juli 2016 · Control experiments in Thy1/ChR2-YFP mice showed that CSD was not induced by pulses of red (630 nm) light that do not activate ChR2 or by light-induced rises in cortical temperature (Supplementary Figure 3(a) to (c)). Characteristics of CSD change when repeated after 20 min.
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WebbAs expected with the Thy1 promoter, there was little expression in the striatum ( Figure 1 A), but at higher magnification, there was strong colocalization between ChR2-YFP and neurofilament (Nflm) in corticostriatal white matter tracts, but no significant colocalization between ChR2-YFP and neuronal nuclei (NeuN) or with the astrocyte marker … WebbNational Center for Biotechnology Information fewer jams will mean
Thy1-Expressing Neurons in the Basolateral Amygdala …
Webb1 dec. 2024 · We utilized Thy1-ChR2-YFP transgenic mice, in which channelrhodopsin 2 … Webb4.光遗传学激活和抑制在进行光遗传学实验之前,兴奋性的转基因动物Thy1-ChR2-YFP或者注射了光遗传学相关的病毒的动物需要先进行行为学训练。 实验时,在麻醉状态下,两根光纤被置于小鼠双侧听觉皮层的第5层或者内侧膝状体表面。 麻醉过后至少两小时再开始进行小鼠行为学实验。 光刺激由473纳米的蓝光或者593纳米的黄光组成,时长100毫秒。 每次光 … Webb15 jan. 2024 · Thy1-ChR2-YFP, line 18, mice were obtained from Jackson Laboratory (B6.Cg-Tg (Thy1-COP4/EYFP)18Gfng/J, stock number 007612). Five adult mice, ranging from 8 to 12 months, were sacrificed using Euthasol (Virbac, Fort Results Expression patterns of Thy1-ChR2 and other Thy1 transgenes have been observed to differ between … deluxe lofted cabin finished interior